Brookfield and the Qatar Investment Authority have invested £400m in Canary Wharf Group.
The funds, split between a £300m equity subscription and £100m revolving credit facility, will be used to complete CWG’s strategic repositioning.
Proceeds will also be used to build out additional residential and life sciences projects on the estate.
The deal comes after CWG secured £309m to refinance its Newfoundland residential tower in March.
Canary Wharf Group Investment Holdings recorded £3.3bn of net assets as of June this year, along with £312m cash on its balance sheet. It also has £130m in available and undrawn commitments, held in revolving credit facilities with its relationship banks.
CWG chief executive Shobi Khan said: “This investment underscores confidence in our business plan and the ongoing strategic repositioning of Canary Wharf.
“We have transformed the estate into a thriving, sustainable mixed-use neighbourhood with award-winning homes, an abundance of amenities and parks, a strong and unique retail offer, and a diverse office portfolio with an expanding life sciences offer. We are confident of the opportunities ahead.”
Brian Kingston, chief executive of Brookfield Real Estate, said: “Canary Wharf continues to evolve into a vibrant and diverse estate well-positioned to meet the needs of the future.”
Kingston added that as a long-term, patient investor with “flexible balance sheet capital and half a century of transitioning mixed-use assets into iconic urban districts”, Brookfield will continue to support CWG “on its journey” and believes “the estate holds significant long-term value creation potential”.
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